Improved method for the PCR-based gene disruption inSaccharomyces cerevisiae

Improved gene disruption method and Cre-loxP mutant system for multiple gene disruptions in Hansenula polymorpha.

In H. polymorpha, there is still a lack of a highly efficient gene disruption method. To help address this issue, we presented a simple and efficient method for both single and multiple gene disruptions in H. polymorpha. The knockout system combined a variation of sticky-end polymerase chain reaction method (SEP), split marker deletion method, co-transformation of single-stranded DNA and mutant...

Disruption of GAL1 Gene in Saccharomyces cerevisiae Leads to Higher Ethanol Production

An improved method for detecting faecal Vibrio cholerae by PCR of the toxin A gene.

A method for removing inhibitor(s) of the PCR assay for the direct detection of cholera toxin A gene (ctxA) in human faeces is described. Inhibitors of the PCR were removed by centrifugation and the activity of the remaining inhibitors by dilution. Based on these data, a protocol was developed for pre-treatment of stool specimens for PCR assay, and a simple and rapid protocol was constructed fo...

An improved collocation method based on deviation of the error for solving BBMB equation

In this paper, we improve b-spline collocation method for Benjamin-Bona-Mahony-Burgers (BBMB) by using defect correction principle. The exact finite difference scheme is used to find defect and the defect correction principle is used to improve collocation method. The method is tested on somemodel problems and the numerical results have been obtained and compared.

PCR- and ligation-mediated synthesis of marker cassettes with long flanking homology regions for gene disruption in Saccharomyces cerevisiae.

We developed a novel method for synthesizing marker-disrupted alleles of yeast genes. The first step is PCR amplification of two sequences located upstream and downstream of the reading frame to be disrupted. Due to the addition of non-specific single A overhangs by Taq DNA polymerase, each PCR product can be ligated with a marker DNA which has T residues at its 3' ends. After amplification of ...